Journal: PLOS One

Article Title: Identification of novel antiviral host factors by functional gene expression analysis using in vitro HBV infection assay systems

doi: 10.1371/journal.pone.0314581

Figure Lengend Snippet: Primers used in this study. Primers used in custom TaqMan® Array Fast plate.

Article Snippet: 35 , GPX2 , Human , FAM , Hs01591589_m1 , Applied Biosystems.

Techniques:

Journal: Frontiers in Physiology

Article Title: Multidrug Resistance-Associated Protein 2 Deficiency Aggravates Estrogen-Induced Impairment of Bile Acid Metabolomics in Rats

doi: 10.3389/fphys.2022.859294

Figure Lengend Snippet: Pre-designed TaqMan ® Gene Expression Assay kits (Life Technologies) used for quantitative reverse transcription-PCR (qRT-PCR).

Article Snippet: Gpx2 , , Rn00822100_gH.

Techniques: Gene Expression

Journal: Frontiers in Physiology

Article Title: Multidrug Resistance-Associated Protein 2 Deficiency Aggravates Estrogen-Induced Impairment of Bile Acid Metabolomics in Rats

doi: 10.3389/fphys.2022.859294

Figure Lengend Snippet:

Article Snippet: Gpx2 , , Rn00822100_gH.

Techniques: High Performance Liquid Chromatography, Chromatography

Journal: International Journal of Medical Sciences

Article Title: Iodine Alters Gene Expression in the MCF7 Breast Cancer Cell Line: Evidence for an Anti-Estrogen Effect of Iodine

doi:

Figure Lengend Snippet: MCF7 cells were treated with Lugol's iodine solution or vehicle alone for 48 hr (see Methods and Materials for details). RNA was isolated and subjected to Microarray Analysis (see Methods and Materials for details). 29 genes were upregulated ≥ 2.0-fold (A) and 14 genes were down regulated ≥ 2.0-fold (B) in response to treatment. Genes were than clustered into functional categories using the DAVID Bioinformatics Database Gene Functional Classification Tool (NIAID/NIH). The fold change in expression is relative to control cells. Bold genes were verified by qRT-PCR (Figure 2 ).

Article Snippet: Probes and Assay ID included Cytchrome P450 1A1 (CYP1A1; Hs00153120_m1), Cytochrome P450 1B1 (CYP1B1; Hs00164383_m1), Aldo-keto Reductase 1C1 (AKR1C1; Hs00413886_m1), Glutathione Peroxidase 2 (GPX2; Hs00702173_s1), Growth Arrest and DNA-Damage-Inducible α (GADD45A; Hs00169255_m1), trefoil factor 1 (TFF1; Hs00170216_m1), GDNF family receptor alpha 1 (GFRA1; Hs00237133_m1), Cyclin D1 (CCND1; Hs00277039_m1), v-myb myeloblastosis viral oncogene homolog (avian)-like 2 (MYBL2; Hs00231158_m1), and WNT1 inducible signaling pathway protein 2 (WISP2; Hs00180242_m1).

Techniques: Isolation, Microarray, Functional Assay, Expressing, Control

Journal: Oncogene

Article Title: CBX3 promotes multidrug resistance by suppressing ferroptosis in colorectal carcinoma via the CUL3/NRF2/GPX2 axis.

doi: 10.1038/s41388-025-03337-9

Figure Lengend Snippet: Fig. 6 CBX3 promotes multidrug chemoresistance by activating the NRF2-GPX2 signaling pathway in vivo. A CBX3 overexpression increased Irinotecan resistance in CRC in vivo. Representative images of tumors, tumor size, and weight were obtained 10 days after the initial tumor cell injection (n = 6). Tumors were measured every two days, and the tumor volumes were calculated. The tumors were excised and weighed 34 days after injection, and representative images of tumors were displayed (bar = 10 mm). B Western blotting to detect the expression of NRF2 and GPX2 in SW480-Con/CBX3 tumor masses with or without Irinotecan treatment. C Immunohistochemical staining of tumor tissues with antibodies against Ki67, NRF2, and GPX2 (low-power view 100×, high-power view 400×, scale bar = 50 μm). D MDA assays in SW480-Con/ CBX3 tumor masses with and without chemotherapy. E CBX3 overexpression increases Oxaliplatin resistance in CRC in vivo. Representative images of tumors, tumor size, and weight were obtained at the indicated time point after the initial tumor cell injection (n = 6). Tumors were measured every two days, and the tumor volumes were calculated. The tumors were excised and weighed 28 days after injection, and representative images of tumors were displayed (bar = 10 mm). A–E Con: SW480-Con cells transplantation tumor masses, CBX3: SW480-CBX3 cells transplantation tumor masses. Data were presented as the means ± SEM, *P < 0.05, **P < 0.01, compared with corresponding control groups.

Article Snippet: SiRNAs, including NRF2 siRNA (h) sc-37030, CUL3 siRNA (h) sc-35130, and GPX2 siRNA (h) sc-62415, were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: In Vivo, Over Expression, Injection, Western Blot, Expressing, Immunohistochemical staining, Staining, Transplantation Assay, Control